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After additional three 10 min washings in TBS with 0.3% (v/v) Tween 20, protein signals were revealed using the Chemiluminescence (ECL) detection system (EuroClone, Pero, Milan, Italy)

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This difference in subcellular localization, combined with its distinct expression patterns, suggests that the two proteins play different roles in regulating cellular GSH levels (Ward et al., 2024)