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We also tested a non-CRC model, the osteosarcoma U2OS cell line, which is wild-type for APC, KRAS and p53, and confirmed a similar 5FU-driven change in mitochondrial mass and morphology (Extended Data Fig

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There was no change in viability between the control and CSE exposed cells at any timepoint (data not shown), which supports cells actively exporting GSH rather than it being released due to cell lysis

This intersection defines a promising biochemical synergy capable of modulating PCOS at its molecular core rather than merely attenuating symptoms