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Briefly, the lentiviral vector containing inserts of interest and three packaging system plasmid (Rev,Gag and VSVG) were mixed and transfected into 293 T cells with EndoFectin-Lenti reagent (GeneCopoeia, USA), and then the virus containing culture medium was collected 48 h after transfection and spun for 10 min at 3000 r.p.m

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With respect to the third concern of our initial findings regarding the spin trapping of a radical associated with A42, this peptide has His moieties at residues 6, 13, and 14