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The PVDF membranes were incubated with primary antibodies overnight at 4 C, incubated with secondary antibodies at room temperature for 1 h, and visualized using the Bio-Rad ChemiDoc XRS + system

Knowles, S

It is worth noting that several amino acid transporters, such as SNAT2, SNAT9, and PAT4 are considered transceptors and shown to regulate mTORC1 independent of their transport function 54,55