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Subjects/Methods: A 1-month pilot clinical study of oral liposomal GSH administration at two doses (500 and 1000 mg of GSH per day) was conducted in healthy adults

After further washing with TBST buffer, the membrane was photographed using the chemiluminescent imaging system

doi: 10.1007/s12011-022-03291-7 130 LeussinkBTNagelkerkeJFvan de WaterBSlikkerveerAvan der VoetGBSrinivasanAet al

The conditions used were: 5 min at 95C and 40 cycles of: 1 min at 95C, 1 min at 55C and 30 s at 72C, followed by a melting curve (81 cycles of 10 s starting at 5C), which allows monitoring the dissociation kinetics of the amplified fragments