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Also, the content of ABA in both mutants and wild-type plants was no significant difference, accompanied by lower expression of ABI3 and ABI4 in AtGLR3.4 mutants under NaCl stress ( Arabidopsis , besides AtGLR3.4, AtGLR3.7 also is involved in the regulation of seed germination under NaCl stress ( Arabidopsis by affecting calcium signaling pathways

Mori S, Kiuchi S, Ouchi A, Hase T, Murase T

After stirring at the same temperature for 30 minutes, the calcium oxalate precipitate was removed by centrifugation and the clear supernatant solution obtained was slowly diluted with 75 ml of acetone with stirring at a temperature of + 4 C