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Each needle puncture through the rubber stopper introduces microscopic particles and dulls the needle tipalways use a fresh sterile syringe for each draw to maintain solution purity

Early synaptic dysfunction or synaptopathy at the level of inhibitory interneurons within the entorhinal cortex and CA3 hippocampal subregion leads to hyperactivity of pyramidal cells, which appears to play a role in the progression of AD neuropathology (e.g., tauopathy) (271, 272)

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In the intracellular pathway, metal ions are taken up by microbial cells and turned into NPs within the cells in the presence of cellular enzymes, whereas in the extracellular process, metal ions are trapped outside the cell surface and reduced by cellular enzymes to form NPs (68)