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Untreated, Lyme disease can become a severely debilitating disease affecting joints, the heart, the brain, and/or the central nervous system

Rotate injection sites (abdomen, thighs, upper arms) to reduce local irritation [8]

In most cases, you'll do this in combination with any medicines your doctor prescribes

To prepare paraffin sections, the fixed brain tissue was dehydrated with 70, 80, 95 and 100% alcohol followed by transparent with xylene, embedded in paraffin and cut into 5 m thickness with a microtome (Leica, Germany)