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immunized macaques with four B-cell peptide epitopes of the SARS-CoV S protein and demonstrated that while three peptides elicited antibodies that protected macaques from viral challenge, one of the peptide vaccines induced antibodies that enhanced infection in vitro and resulted in more severe lung pathology in vivo 67

Hwang S, Song S, Hong YK, Choi G, Suh YS, Han SY, et al

Mol Biosyst 12(8):23592372 Kuehn JS, Gorden PJ, Munro D, Rong R, Dong Q, Plummer PJ et al (2013) Bacterial community profiling of milk samples as a means to understand culture-negative bovine clinical mastitis

For double transductions (GLUT3 allele + shGLUT3), unmodified THP-1 cells were serially transduced first with the GLUT3 expression plasmid by puromycin selection followed by the shGLUT3 allele by hygromycin selection