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In summary, cells were grown at 30 C to mid-log, induced with 500 M IPTG and grown for an additional 5 h

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Measures of Disease Frequency of 2050 cases per 100,000 persons Female-to-male ratio of 8:1 Onset usually at 2040 years of age In children, the majority of cases occur after 11 years of age

Changes in the epigenetic landscape of monocytes leading to BDG-induced trained immunity are mediated by the activation of mechanistic target of rapamycin (mTOR) and hypoxia-inducible factor 1 (HIF-1)