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Initially (untreated cells for initial time point) or after 4 days (with varying treatment conditions), 40 L of Cell Titer Glo reagent (Promega) was added to each well, mixed briefly, and the luminescence read on a luminometer (Molecular Devices)

It encompasses several pathways, including neurological (vagus nerve), hormonal, immunological, and microbiome-mediated signals, all of which play a critical role in maintaining physiological homeostasis and responding to systemic stresses (Sasso et al., 2023)

1 ug of RNA was reverse transcribed using the Superscript III Reverse Transcriptase kit (Invitrogen) according to the manufacturers instructions

Late effects of low-dose radiation on the bone marrow, lung, and testis collected from the same exposed BALB/cJ mice