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After washing, sections were incubated with fluorescent secondary antibodies for 1 h at room temperature, washed again, mounted, and imaged using Olympus laser scanning confocal microscope (Spin SR) or slide scanner of Zeiss (Axioscan 7)

Finally, several inorganic and organic molecules, such as sodium arsenite, triphenylphosphine (Fig

Beads were washed with 20 mM and 40 mM imidazole steps and protein was eluted using 250 mM imidazole in 50 mM Tris-HCl, 100 mM NaCl, 0.5 mM MgCl 2 (pH 7.5)