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Description

10.1248/bpb.19.1479 9 BaswanS

pI of a protein could be determined by several experiment methods, but an approximate value could be calculated by mathematics methods

An Agilent 1100 binary pump was used to generate the HPLC gradient as follows: 05% B for 5 min, 545% B for 40 min, 4580% B for 3 min and then to 5% B in 2 min (A = 0.1 M acetic acid in water