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Graph shows P2RY8 ligand bioassay of the SAS supernatant and methanol extracts from the protein precipitate, as indicated by arrows ( n = 4)

Immunofluorescence Thin sections of mouse brain and eyeball were washed with PBS, permeabilized with PBS containing 0.05% (v/v) saponin and 5% (v/v) normal goat serum for 3 min, and treated with PBS containing 1.5% normal goat serum for 1 h to block nonspecific binding

The other portion of undifferentiated TA cells transfer to the bulge and outer root sheath and then back to the bulge during the catagen phase to colour the hair during the next hair follicle cycle [212]