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10.1007/s12975-020-00844-7 88

Activation of GSK3 prevents termination of TNF-induced signaling

For immunoprecipitation and pulldown assays, cells were rinsed once in ice-cold PBS and lysed either in 0.3% CHAPS lysis buffer [40 mM HEPES pH 7.4, 120 mM NaCl, 1 mM EDTA and 0.3% (w/v) CHAPS (3-((3-cholamidopropyl) dimethylammonio)-1-propanesulfonate)] or in 1% Triton X-100 lysis buffer (20 mM Tris/HCl pH 8.0, 100 mM NaCl, 1 mM EDTA and 1% (v/v) Triton X-100) supplemented with 1 g/ml pepstatin A, 1 g/ml aprotinin and 100 mM PMSF (phenylmethylsulfonyl fluoride) on ice for 20 min