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Figure 4 3.4 Confirmation of essentiality of genes and immunogenicity of knockouts in calf trial 3.4.1 Bacteria shedding in fecal matter The MAP culture qPCR indicated that all the inoculated animals had passive MAP shedding 24 hours after inoculation

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Increased fecal microbiota diversity and altered composition Fecal microbiome diversity (-diversity), as assessed by 16S sequencing, increased by W12 (p=0.021) and remained higher than baseline at subsequent W24 and W52 timepoints (Fig

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