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Afterwards, the membranes were incubated at 4C overnight under constant shaking with the following primary antibodies, diluted in 5% skimmed milk-containing TTBS: anti-ACSL4 (Abcam, ab110007, 1:1000), anti-Cyclin A2 (Cell Signaling Technology, 67955, 1:1000), anti-DPP4 (Cell Signaling Technology, 67138, 1:1000), anti-GPX4 (Abcam, ab125066, 1:1000), anti--Actin (Merck, A5441, 1:10000), anti-VCP (Cell Signaling Technology, 2648, 1:1000)

Influence of plasma on GSH in the presence of iron complexes As the last step the impact of plasma treatment on GSH and GSSG in the presence of iron(II) and iron(III) complexes was investigated

One method that has gained popularity is Intravenous (IV) Infusion Therapy