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and isolating the acetyl-L-carnitine nitrate, wherein reacting L-carnitine with acetic anhydride to produce acetyl-L-carnitine free base comprises dissolving the L-carnitine in acetic acid, and wherein the reaction between L-carnitine and acetic anhydride occurs at a temperature of between 30 C

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That increased TCA activity elicits ER stress does not necessarily portend cellular dysfunction

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