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Some animal studies, such as one involving a segmental bone defect in rabbits (Sebecic et al., 1999), explored its local application

Regulatory agencies require extensive clinical evidence before approval, and the absence of such data means safety and effectiveness remain uncertain

Given that some women may already suffer from IDA prior to pregnancy due to factors such as dietary imbalance and unhealthy lifestyle habits (14, 23, 24), and their condition tends to worsen during pregnancy, thereby increasing pregnancy-related risks, based on existing pharmacological experimental foundations (4, 25, 26), we adopted a method of feeding rats with a simple iron-deficient diet to induce IDA before mating, thus establishing a pregnant rat model under IDA conditions