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The fixed cells were stained with 1 g mL 1 propidium iodide (PI) (catalog #P4170-25MG, Sigma-Aldrich) and the cell cycle distribution was analyzed with a UV-LSR flow cytometer, by measuring the red fluorescence of the PI-stained DNA content

The chemokines they release, such as CXCL1 and CXCL2, attract monocytes and neutrophils to the liver, where they contribute to the localization and amplification of inflammatory responses [180]