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Identification of novel L2HGDH mutation in a large consanguineous Pakistani family- a case report

Wash hands thoroughly Clean the tops of both vials with alcohol swabs Draw your chosen solvent into a sterile syringe Inject the solvent slowly into the IGF-1 LR3 vial, directing the stream against the glass wall rather than onto the powder Let the vial sit undisturbed for several minutes, allowing the peptide to dissolve naturally Gently swirl to mix, never shake vigorously Confirm the solution is clear with no visible particles Label with date and concentration For a 1 mg vial, adding 1 mL of solvent creates a concentration of 1000 mcg/mL

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The absorbance of the resultant solution was measured at 532 nm and compared to the blank sample