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These cells remain metabolically active, adopt a distinct phenotype (including chromatin changes), and critically secrete a variety of pro-inflammatory cytokines, proteases, growth-factors and extracellular-matrix modifying signals

Then, sodium dodecyl sulfatepolyacrylamide gel electrophoresis (NCM Biotech, Jiangsu, China) was put into use for separating proteins, and these were transferred into polyvinylidene difluoride membranes (Millipore, USA) by an electro-blotting apparatus

The browning was reduced by 75% by lowering the storage temperature from 25C to 3C (124)