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An equimolar concentration of Cu(II)acetate was added to HSA in 50 mM Hepes, 50 mM NaCl, pH 7.4 and the sample was incubated 10 min at room temperature, which is sufficient for CuHSA complex formation (in separate kinetic experiment we established that the half-life of Cu(II) binding to HSA is approx

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Quantitative lipidomics analysis has revealed that PUFA-PL containing arachidonic acid or adrenaline acid is critical and is oxidized to PL-PUFA-OOH via the Fenton reaction, thereby driving ferroptosis