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Results GSH depletion induces cell death in RPE cells To evaluate the effect of GSH depletion on the RPE cells, we used three different approaches: depletion of Cys 2 from cell culture media, treatment with buthionine sulphoximine (BSO, 1000 M) to block de novo synthesis of GSH, or treatment with erastin (10 M), an inhibitor of system x c 1

169 Molecular mediators that drive organ crosstalk Interorgan crosstalk among the kidney, heart, brain, adipose tissue, liver, skeletal muscle, pancreas, and intestine plays a pivotal role in the development of insulin resistance and -cell dysfunction, which are central to the progression of diabetic complications

By directly scavenging reactive oxygen species (ROS), it helps to prevent mitochondrial dysfunction, supports ATP synthesis, and inhibits the pathways leading to cell death, including apoptosis

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