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Ussing chamber analysis On day 32 post-airlift, four replicate ALI cultures from each KO treatment (4 donors) were subjected to electrophysiological analyses in an Ussing Chamber (Physiologic Instruments) under open-circuit conditions, where intermittent short-circuit current measurements with pulsing (200 ms pulses at 5 mV) was performed to obtain resistance and conductance values

Its important to remember that the effects of smoking on semen production are complex and not fully understood