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Thirdly, the AVONEX trial was the first to include the 9-Hole Peg Test, Timed 25-Foot Walk, and the Paced Serial Addition Test, which later were incorporated into the Multiple Sclerosis Functional Composite (MSFC), recommended as a new clinical outcome measure by the National MS Society Task Force on Clinical Outcomes Assessment

Fluorescence was analyzed using a flow cytometer (BD FACSCanto II, BD Biosciences, Franklin Lakes, NJ, USA) equipped with a 488 nm laser for excitation