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Mason SA, Trewin AJ, Parker L, Wadley GD

Samples were prepared by adding 2 or 4 sample buffer (Bio-Rad, Laboratories, Hercules, CA, USA) and boiling for 5 min at 95 C before loading onto the gel

They observed a significant increase in ethylene production of the ERF2/TERF2 overexpression lines and a decrease in the ERF2/TERF2 antisense-lines compared to the wild type, suggesting that these ERFs are positive regulators of ACO3 expression in tomato (Zhang et al., 2009)