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Factors influencing the levothyroxine dose in the hormone replacement therapy of primary hypothyroidism in adults

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Nonspecific binding was blocked with 5% skim milk in Tris-buffered saline with 0.1% Tween 20 buffer (Sigma-Aldrich), and the membrane was incubated with a cocktail of monoclonal antibodies (1:800 dilution) purchased from Mitosciences (Eugene, OR, USA) for evaluation of oxidative phosphorylation complexes, including complex I (CI)-20 kDa subunit (NDUFB8), complex II (CII)-30 kDa subunit (SDHB), complex III-core 2 subunit (CIII)-48 kDa, complex IV-subunit I (CIV-I)-40 kDa, and complex V-subunit alpha (CV)-55 kDa

Schihada, H., Shekhani, R