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2394-C Ubc13 PROTECTS AGAINST DEFECTIVE MITOPHAGY IN NON-ALCOHOLIC FATTY LIVER DISEASE BY PROMOTING K63-LINKED p62 UBIQUITINATION Feng Wang 1 , Yoon-Seok Roh 1 , Jin Lee 2 , Jeong-Su Park 1 , Hwan Ma 1 and Ekihiro Seki 3 , (1)Chungbuk National University College of Pharmacy, (2)University of California, San Diego, La Jolla, CA, (3)Cedars-Sinai Medical Center, Torrance, CA Background: Ubiquitination is a post-translational modification and contributes to mitophagy which plays an essential role in nonalcoholic fatty liver disease (NAFLD)

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Link: Stable protein-protein interactions are easiest to isolate by physical methods like pull-down assays because the protein complex does not disassemble over time