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The baseline metabolic state can significantly influence the responsiveness to 5-Amino-1MQ

Although these effects were demonstrated in differentiated cortical neurons, they can be highly relevant to NSCs, which rely on Shh signaling supporting neurogenesis by maintaining redox balance, protecting mitochondrial integrity, and enabling the maturation and neurite extension of newly generated neurons (Favaro et al., 2009)

Vigorous shaking or vortexing introduces shear stress that denatures peptide structure even before temperature exposure becomes a factor

The lipid extracts were cleared by centrifugation (17,000 g , 20 C, 10 min), and the lipids in the supernatant were analyzed by LC-MS