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2.4 Sample preparation For LCQTOF-MS/MS analysis, 1 mg of powder or homogenized tablet was dissolved or extracted using 5 mL of a 1:1:1 (v/v/v) mixture of water/methanol/acetonitrile, assisted by ultrasonication for 10 min, and afterwards filtered by a Whatman 0.2 m pore size polytetrafluoroethylene (PTFE) filter medium (GE Healthcare, Chicago, IL, United States)

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By using HFD as a nutritional prototype and lesions generated from wild-type (WT) endometria as a genetic phenotype to recapitulate retrograde menstruation in normal women, we provide support for ectopic lesion genotype as an important variable in assessing dietary contributions to endometriosis risk and progression