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The samples and standards (Amino Acid Hydrolysate Standard, Waters Corporation) were derivatized using the AccQ-Tag Ultra Derivatization Kit (Waters Corporation)

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The term oxidative stress, first introduced by Helmut Sies in the late 1980s ( In IBD, evidence suggests that oxidative stress plays a crucial role in the onset and progression of the disease ( Over the past decades, extensive research has been conducted to understand the mechanisms underlying oxidative stress in IBD