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Immunofluorescence Fresh rat gastrocnemius muscle tissues were cryopreserved at 80 C and OCT-embedded 8 weeks after injury for sampling and sectioning

In addition, OAT induces proline dehydrogenase (PRODH), leading to mitochondrial ROS production, which further amplifies TGF-1 signaling and tissue fibrosis [71]

For Syn 1103 and Syn 1103 -104C constructs, eluted fractions were further purified using a HiTrap Heparin HP column (Cytiva, MA, USA), leveraging cation-exchange conditions due to their higher isoelectric point (pI ~9.5)