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Secondary carnitine deficiency (SCD) is triggered by excessive carnitine loss, a couple of metabolic disorders, poor carnitine content in the diet or malabsorption, Fanconi syndrome (free carnitine loss through renal tubule), peritoneal dialysis or elevated excretion of acyl-carnitine (Winter SC, 2003)

l-carnitine supplementation may affect both of these pathways in rat skeletal muscle [22]

Fold change relative to the expression of the sample genes and reference genes is calculated using normalized expression ((Ct)) method with default threshold values using CFX Manager Software (BioRad)

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